rabbit anti mmp12 antibody  (Bioss)

Journal: Pain Research & Management

Article Title: Locating the Site of Neuropathic Pain In Vivo Using MMP-12-Targeted Magnetic Nanoparticles

doi: 10.1155/2019/9394715

Figure Lengend Snippet: Macrophage infiltration and MMP-12 upregulation occurred in injured nerves. (a–d) Representative images of immunohistochemistry staining on consecutive sections for macrophage marker CD68 and MMP-12, in the left L5 spinal nerves distal to the DRG, 1 week after SNL or sham surgery (CD68 or MMP-12: red, nuclei: purple, scale bar = 20 μ m). (e) CD68- and (f) MMP-12-labelled areas were quantified for SNL and sham rats' spinal nerves ( n =5 per group). Statistically significant difference in the left L5 spinal nerve CD68-labelled area was determined by Mann–Whitney U test ( ∗∗ p < 0.01).

Article Snippet: Sections were incubated with 1.5% normal goat serum (AK-5001, Vector Laboratories, CA, USA), before a 2 h incubation with either a mouse monoclonal antibody to CD68 (MAB1435, Merck Millipore, MA) or a rabbit polyclonal antibody to MMP-12 (bs-1854R, Bioss, MA, USA), at a dilution of 1 : 200. biotinylated goat anti-mouse (Ba-2900, Vector Laboratories, CA, USA) or anti-rabbit (AK-5001, Vector Laboratories, CA, USA) IgG antibodies diluted by 1 : 200 in 1.5% normal goat serum were applied for 30 min.

Techniques: Immunohistochemistry, Staining, Marker, MANN-WHITNEY

Journal: Pain Research & Management

Article Title: Locating the Site of Neuropathic Pain In Vivo Using MMP-12-Targeted Magnetic Nanoparticles

doi: 10.1155/2019/9394715

Figure Lengend Snippet: MMP-12 upregulation in ligated left L5 spinal nerve is observed for up to 2 weeks. MMP-12 activity of spinal nerves at 1 ( n =5) or 2 ( n =4) weeks after SNL and 1 week after sham surgery ( n =5) was compared. Statistically significant differences between groups for each spinal nerve were determined by Mann–Whitney U test ( ∗ p < 0.05).

Article Snippet: Sections were incubated with 1.5% normal goat serum (AK-5001, Vector Laboratories, CA, USA), before a 2 h incubation with either a mouse monoclonal antibody to CD68 (MAB1435, Merck Millipore, MA) or a rabbit polyclonal antibody to MMP-12 (bs-1854R, Bioss, MA, USA), at a dilution of 1 : 200. biotinylated goat anti-mouse (Ba-2900, Vector Laboratories, CA, USA) or anti-rabbit (AK-5001, Vector Laboratories, CA, USA) IgG antibodies diluted by 1 : 200 in 1.5% normal goat serum were applied for 30 min.

Techniques: Activity Assay, MANN-WHITNEY

Journal: Pain Research & Management

Article Title: Locating the Site of Neuropathic Pain In Vivo Using MMP-12-Targeted Magnetic Nanoparticles

doi: 10.1155/2019/9394715

Figure Lengend Snippet: MMP-12-targeted probe is selectively cleaved by MMP-12 in vitro . (a) U87 glioma model MRI phantom showed lower intensity in the ROI (indicated by yellow circle) of (2) cells treated with probe only, compared to (3 and 4) cells treated with probe and MMP408, (5) untreated cells, and a (1) blank. (b) Microscopic observation of U87 cells stained with prussian blue, following incubation with the probe, shows iron-positive areas (scale bar = 100 μ m).

Article Snippet: Sections were incubated with 1.5% normal goat serum (AK-5001, Vector Laboratories, CA, USA), before a 2 h incubation with either a mouse monoclonal antibody to CD68 (MAB1435, Merck Millipore, MA) or a rabbit polyclonal antibody to MMP-12 (bs-1854R, Bioss, MA, USA), at a dilution of 1 : 200. biotinylated goat anti-mouse (Ba-2900, Vector Laboratories, CA, USA) or anti-rabbit (AK-5001, Vector Laboratories, CA, USA) IgG antibodies diluted by 1 : 200 in 1.5% normal goat serum were applied for 30 min.

Techniques: In Vitro, Staining, Incubation

Journal: Pain Research & Management

Article Title: Locating the Site of Neuropathic Pain In Vivo Using MMP-12-Targeted Magnetic Nanoparticles

doi: 10.1155/2019/9394715

Figure Lengend Snippet: Coronal T2-WI a day after intrathecal injection of MMP-12-targeted probe. White arrows indicate left and right L5 spinal nerves, in sham and SNL rats ( n =2 per group). Compared to uninjured nerves, injured nerves are enlarged, and iron-induced signal loss is observed at the approximate site of ligation. (a) Sham 1. (b) Sham 2. (c) SNL 1. (d) SNL 2.

Article Snippet: Sections were incubated with 1.5% normal goat serum (AK-5001, Vector Laboratories, CA, USA), before a 2 h incubation with either a mouse monoclonal antibody to CD68 (MAB1435, Merck Millipore, MA) or a rabbit polyclonal antibody to MMP-12 (bs-1854R, Bioss, MA, USA), at a dilution of 1 : 200. biotinylated goat anti-mouse (Ba-2900, Vector Laboratories, CA, USA) or anti-rabbit (AK-5001, Vector Laboratories, CA, USA) IgG antibodies diluted by 1 : 200 in 1.5% normal goat serum were applied for 30 min.

Techniques: Injection, Ligation